The Poplar DHQDr qPCR primers shown above contain cross dimers with a G of 0.7 kcal/mol, and 1 GC clamp, no other secondary structures are reported. Targets were quantified with specific primer pairs designed with the Beacon Designer 4.0 (Premier Biosoft International, Palo Alto, CA, USA). The author company, Premier Biosoft has also launched a simple version for evaluation of oligos for real time PCR primers and probes (support limited to TaqMan and SYBR Green primers) by the name Beacon designer free version. Secondary structures are given for primers and primer pairs, along with the estimated Gibbs free energy required to break the bonds formed. Avoidance of the cross homology renders specificity to the primers in question and they are highly targeted and only amplify the intended sequence and no other targets.īeacon Designer has been used in workshops and conferences related to real time PCR study as a reference software.
#PREMIER BIOSOFT BEACON DESIGNER 8.02 VERSIONS SOFTWARE#
The software designs real time pcr primers and probes for complex differential gene expression and allele discrimination assays.
The size of the latest downloadable setup file is 81.4 MB. The following versions: 2.7, 2.6 and 2.5 are the most frequently downloaded ones by the program users. The software currently supports the following real time PCR chemistries for efficient primer and probe design.īeacon Designer program has built in support for homology avoidance using the standard web BLAST service or the set-up of a desktop BLAST feature for identification of regions that are significantly cross homologous with the target gene sequence. The actual developer of the program is PREMIER Biosoft International. It is compatible to work on Windows as well as on Mac.
Beacon Designer designs highly specific and efficient primers and probes for real time PCR ( polymerase chain reaction) assays.
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